BP Fluor 568 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. BP Fluor 568 TCO is comparable to Alexa Fluor 568 TCO. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date. The half-life of TCO is short as it will naturally isomerize into CCO (cis-cycloctene) and lose the reactivity. TCO compound is not recommended for long term storage. Alexa Fluor is registered trademarks of Thermo Fisher Scientific.
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