Anti-mouse TIGIT / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone 1F4 / 29F.1A12.1)

The rat anti-mouse PD-1 monoclonal antibody 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein. The 1F4 antibody binds to the TIGIT protein (WUCAM, Vstm3 or VSIG9), a novel immune checkpoint receptor with inhibitory function. TIGIT is expressed on T cells and natural killer (NK) cells, and several human cancers, including melanoma, NSCLC, and colorectal cancer. Similar to PD-1, the TIGIT receptor limits antitumor immune response in cancer. It is under investigation how the recombinant anti-mouse PD-1 / TIGIT bispecific antibodies (29F.1A12.1 / 1F4) behave different from the individual monoclonal antibodies and their combination. This recombinant anti-mouse PD-1 / TIGIT bispecific antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: 29F.1A12) and the mouse anti-mouse TIGIT antibody (hybridoma clone name or number: 1F4).

Product Details
Species Reactivity	Mouse
Source	Mammalian cells
Host/Isotype	Mouse IgG2c, kappa
Class	Bispecific Antibody
Type	In Vivo Grade Recombinant
Immunogen	The original rat hybridoma (clone name: 10F.9G2) was generated by immunizing rats with the mouse PD-L1 cDNA and mouse PD-L1 CHO transfectants. The original rat hybridoma (clone name: RMP1-14) was generated by immunizing rats with mouse PD-1-transfected BHK cells.
Clone	1F4, the same variable region sequences as the mouse anti-mouse TIGIT monoclonal antibody (clone number: 1F4). 29F.1A12.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Conjugate	Unconjugated
Purity	>95% by SDS-PAGE under reducing conditions
Storage Condition	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Storage Buffer	0.2 μM filtered solution of 1x PBS