Anti-mouse PD-L1 / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone 10F.9G2.1 / RMP1-14.1)

The rat anti-mouse PD-1 monoclonal antibody (clone No. RMP1-14, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein. The in vivo grade recombinant rat anti-mouse PD-1 / PD-L1 bispecific antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells. The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein. It is under investigation how the recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (RMP1-14 / 10F.9G2 and 10F.9G2 / RMP1-14) behave different from the individual monoclonal antibodies and their combination. This recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: RMP1-14) and the rat anti-mouse PD-L1 antibody (hybridoma clone name or number: 10F.9G2).

Product Details
Species Reactivity	Mouse
Source	Mammalian cells
Host/Isotype	Mouse IgG2c, kappa
Class	Bispecific Antibody
Type	In Vivo Grade Recombinant
Immunogen	The original rat hybridoma (clone name: 10F.9G2) was generated by immunizing rats with the mouse PD-L1 cDNA and mouse PD-L1 CHO transfectants. The original rat hybridoma (clone name: RMP1-14) was generated by immunizing rats with mouse PD-1-transfected BHK cells.
Clone	10F.9G2.1, the same variable region sequences as the rat anti-mouse PD-L1 monoclonal antibody (clone number: 10F.9G2). RMP1-14.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14).
Conjugate	Unconjugated
Purity	>95% by SDS-PAGE under reducing conditions
Storage Condition	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Storage Buffer	0.2 μM filtered solution of 1x PBS