Protein A-DM1

Protein A-DM1 is a Protein-drug conjugate (PDC) of Protein A with with microtubule-inhibiting drug DM1 (Mertansine) conjugate through a linker. >95% protein A were conjugated with DM1, the DPR (drug protein ratio) n = 1-3 drugs per protein. Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. The protein is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain can bind proteins from many mammalian species, most notably IgGs (immunoglobulins). Protein A has the remarkable ability to bind to the Fc region of most immunoglobulins. It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. DM1 (Mertansine) is a microtubule-inhibiting drug that is a synthetic derivative of maytansine and is used as a potential cancer treatment. DM1 can be attached to a monoclonal antibody or proteins with a linker to create a conjugate that is developed to overcome systemic toxicity associated with maytansine and to enhance tumor-specific delivery. DM1 is a strong antiproliferative chemotherapeutics toward over 60 types of cancer cell lines. This product is for research use only.

Product Details
Species Reactivity	Bind to the Fc region of most immunoglobulins
Source	E. coli
Type	Recombinant Protein
Measurement	A275 of 0.1% solution: ~0.149
Isoelectric point (pI)	4.7-4.8
DAR (Drug to Antibody Ratio)	>95% protein conjugated, 1-3 drugs per protein
Conjugate	Protein A conjugated with -GGFG-Exatecan (DX-8951)
Purity	>95%
Molecular Weight	~44,600 (Apparent MW by SDS-PAGE: 45,000)
Protein Concentration	1 mg/ml
Formulation	Liquid
Storage Condition	4°C for short time, -20°C or -80°C for long time.
Storage Buffer	20 mM Sodium Borate, 6% Trehalose, pH8.0