DusQ 3 NHS ester is a dark, far-red nonfluorescent DusQ 3 derivative for labeling with the primary amines of proteins, modified oligonucleotides. The labeling with covalent amide bond occurs most efficiently at pH 7-9. DusQ 3 is used as an acceptor for preparation of fluorogenic dual-labeled probes, for qPCR, sequencing and other fluorescence-quenched system applications. Due to quenching range characterised as 620-730 nm DusQ 3 is useful as an acceptor in fluorescence resonance energy transfer (FRET) combined with far-red to near-IR emitting dyes such as Cyanine5, Cyanine5.5, AF 647.
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