Diazo-PEG3-TCO consists of a diazo group for efficient release of captured molecules and three PEG units for enhanced water-solubility. TCO is a click chemistry handle which readily reacts with tetrazine groups on a target molecule. The half-life of TCO is short as it will naturally isomerize into CCO (cis-cycloctene) and lose the reactivity. TCO compound is not recommended for long term storage.
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