Sulfo-NHS-LC-Biotin

BroadPharm MagicLink™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines. Features:

• Amine-reactive: reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble charged: sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length spacer arm: (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
• Irreversible forms: permanent amide bonds; spacer arm cannot be cleaved
• Cell surface: labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Protein labeling: biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes

Sulfo-NHS-LC-Biotin is water-soluble, non-cleavable, and enables simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.
Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits