Sulfo-NHS-Biotin Kit

MagicLink™ Sulfo-NHS-Biotin is a water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Sulfo-NHS-Biotin is the shortest arm length (13.5 angstroms) of three MagicLink™ biotinylation reagent which is water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is offered in separate package sizes and as complete protein labeling kits.

Features:

• Amine-reactive: reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
• Soluble charged: sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Protein labeling: biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface: labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Irreversible forms: permanent amide bonds; spacer arm cannot be cleaved
• Very short spacer arm: (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.