NHS-PEG4-Biotin

MagicLink™ NHS-PEG4-Biotin is a long (29.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amine-containing macromolecules and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.
Features:

• Amine-reactive: reacts with primary amines (-NH2)
• Pegylated spacer arm: hydrophilic polyethylene glycol (PEG) spacer arm (29 ), this reduces steric hindrance when binding to avidin molecules
• Protein labeling: biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Enhances solubility: pegylation enhances water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible forms: permanent amide bonds; spacer arm cannot be cleaved