The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with Mouse Anti β-Tubulin IgG, and, after washing, was further incubated with biotin-conjugated Goat Anti-Mouse IgG Biotin Conjugate. Finally, it was rinsed with PBS and stained with Streptavidin FITC Conjugate at 5 μg/mL (shown in green) and DAPI-2HCl at 1 μg/mL (shown in blue)
Usually ships within 24 hours.
CWR22R cells were fixed with 4% paraformaldehyde, blocked by 3% BSA. Subsequently the specimen was cultured at 4°C overnight with Anti PSMA monoclonal antibodies (1 : 100), and incubated with FITC-conjugated anti mouse IgG antibody at room temperature for 2h. DAPI nucleic stain was performed in blue.