- Western blot
- flow cytometry
- immunocytochemical assays, including In-Cell Western? assays and On-Cell
- Western cell-based assays
- protein arrays
- microscopy
- tissue section imaging
- in vivo imaging and optical probe development
800CW NHS Antibody Labeling Kit
Catalog #: bp-50030
Key Features and Details
tumour-specific NIRF imaging of COC183B2-800 in a subcutaneous mouse tumour model.
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Overview
800CW (IRDye 800CW acid equivalent) is a near-infrared fluorescent dyes. The unique structure has 4 sulfonates which make it as a high water soluble and less aggregation in aqueous solution. 800CW is used for protein and antibody labeling, or nucleic acid applications with high labeling density.
800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye based on its enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios. -
Components
- active Dye, 1
- Buffer, 1
- Desalt column, 1
- DMSO, 1 rxn 1 mL, 1
- NaN3 3%, 0.5 mL, 1
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Application
800CW dye can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below. -
Images
tumour-specific NIRF imaging of COC183B2-800 in a subcutaneous mouse tumour model.
Immunohistochemistry flat-bed fluorescence (A) and fluorescence microscopy (B and C) images of pancreatic tissue of mouse injected with exendin-4- 800CW. Marti Boss et al. J Nucl Med 2020;61:1066-1071
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Protocol
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Reference
- Marshall, M.V., Draney, D., Sevick-Muraca, E.M. et al. Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats. Mol Imaging Biol 12, 583 594 (2010)
- Kijanka, M., Warnders, FJ., El Khattabi, M. et al. Rapid optical imaging of human breast tumour xenografts using anti-HER2 VHHs site-directly conjugated to IRDye 800CW for image-guided surgery. Eur J Nucl Med Mol Imaging 40, 1718 1729 (2013).
- Ruimin Huang, Jelena Vider, Joy L. Kovar, D. Michael Olive, Ingo K. Mellinghoff, Philipp Mayer-Kuckuk, Moritz F. Kircher and Ronald G. Blasberg;Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma ;Clin Cancer Res October 15 2012 (18) (20) 5731-5740;
- Linder, Karen E., et al. "Synthesis, in vitro evaluation, and in vivo metabolism of fluor/quencher compounds containing IRDye 800CW and Black Hole Quencher-3 (BHQ-3)." Bioconjugate chemistry 22.7 (2011): 1287-1297.
- Bujie Du, Xingya Jiang, Yingyu Huang, Siqing Li, Jason C Lin, Mengxiao Yu, and Jie Zheng
Tailoring Kidney Transport of Organic Dyes with Low-Molecular-Weight PEGylation
Bioconjugate Chemistry 2020 31 (2), 241-247 - Francisco Romero Pastrana, John M. Thompson, Marjolein Heuker, Hedzer Hoekstra, Carly A. Dillen, Roger V. Ortines, Alyssa G. Ashbaugh, Julie E. Pickett, Matthijs D. Linssen, Nicholas M. Bernthal, Kevin P. Francis, Girbe Buist, Marleen van Oosten, Gooitzen M. van Dam, Daniel L. J. Thorek, Lloyd S. Miller & Jan Maarten van Dijl (2018) Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probe, Virulence, 9:1, 262-272,