Name: BP Fluor 647 NHS Antibody Labeling Kit (1 x 1 mg)
Brand: BroadPharm
SKU: BP-50027
Price: 780.00 USD
Availability: InStock

BP Fluor 647 NHS Antibody Labeling Kit

Catalog #: bp-50027

Key Features and Details

HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 μg/ml BP Fluor 647 anti-Vimentin (clone O91D3) antibody (red) in 5% FBS for two hours at room temperature, followed by BP Fluor 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).

Overview

BP Fluor 647 is a far-red bright and photostable fluorophore absorbed at 650 nm and emitted at 671 nm. BP Fluor 647 is water soluble and pH-insensitive from pH 4 to pH 10. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution.
BP Fluor 647 is spectrally similar to Cy5, Cyanine 650. These three dyes, BP Fluor 647, Cyanine 650, and Cy5, are bright green-fluorescent dye optimal for use with the 633nm, 640nm 650 nm argon laser.
BP Fluor 647 NHS antibody labeling kit is used for protein and antibody labeling, or nucleic acid applications with high labeling density for applications such as immunolabeling, fluorescence microscopy, and flow cytometry.
Components
1. active Dye, 1
2. Buffer, 1
3. Desalt column, 1
4. DMSO, 1 rxn 1 mL, 1
5. NaN3 3%, 0.5 mL, 1
Application
All three dyes can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.
- Flow cytometric analysis of antibody surface-stained cells (FC)
- Flow cytometric analysis of intracellularly-stained cells ( ICFC)
- Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded. (IHC-F)
- IHC-P
- Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies. (ICC)
- Immunofluorescence Microscopy, Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides, or tissue preparations (IF)
- Immunodetection applied to formalin-fixed tissue sections of at least 300 microns in thickness, which are optically cleared by tissue clearing reagent (3-DIHC)
- IHC
- WB
Images

HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 μg/ml BP Fluor 647 anti-Vimentin (clone O91D3) antibody (red) in 5% FBS for two hours at room temperature, followed by BP Fluor 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD64 (NULL.1) BP Fluor 647 antibody prepared by MagicLink™ BP Fluor 647 Mal kit, 4 µl reagent / 100 µl of peripheral whole blood).
Protocol

BP Fluor647 Cy5 Cyanie650 General protocol dye MAL labeling kit General protocol dye NHS labeling kit

BP Fluor 647 NHS Antibody Labeling Kit

BP Fluor 647 NHS Antibody Labeling Kit

Key Features and Details

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Overview

Components

Application

Images

Protocol

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