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Cyanine 650 NHS Antibody Labeling Kit
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Dye Antibody Labeling Kit
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Cyanine 650 NHS Antibody Labeling Kit (1 x 1 mg)
Cyanine 650 NHS Antibody Labeling Kit
Catalog #: bp-50021
Catalog #
Unit
Price
Qty
BP-50023
1 x 100 ug
$260.00
BP-50022
3 x 100 ug
$450.00
BP-50021
1 x 1 mg
$680.00
Usually ships within 24 hours.
Would you like to inquire about custom quantity?
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Overview
These three dyes, BP Fluor 647, Cyanine 650, and Cy5, are bright green-fluorescent dye optimal for use with the 633, 650 nm argon laser. The three dyes have the same fluorophore backbone structure which contributes to the almost identical absorption and emission spectrum. They are only different from the number of soluble groups SO3Na, i.e. Cy5 has two SO3Na groups, and Cyanine 650 has three while BP Fluor 647 has four.
All these dyes are water soluble and pH-insensitive from pH 4 to pH 10. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution. These dyes are used for protein and antibody labeling, or nucleic acid applications with high labeling density for numerous bio applications.
Components
active Dye
Buffer
Desalt column
Application
All three dyes can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.
Flow cytometric analysis of antibody surface-stained cells (FC)
Flow cytometric analysis of intracellularly-stained cells ( ICFC)
Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded. (IHC-F)
IHC-P
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies. (ICC)
Immunofluorescence Microscopy, Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides, or tissue preparations (IF)
Immunodetection applied to formalin-fixed tissue sections of at least 300 microns in thickness, which are optically cleared by tissue clearing reagent (3-DIHC)
IHC
WB
Images
Protocol
BP Fluor647 Cy5 Cyanie650
General protocol dye MAL labeling kit
General protocol dye NHS labeling kit
Other Related Products
Cyanine 650 NHS Antibody Labeling Kit (1 x 100 ug)
Cyanine 650 NHS Antibody Labeling Kit (3 x 100 ug)