This biotin derivative bears a terminal pyridyldithiol moiety. It can be used to prepare various biotinylated conjugates. Biotin can be subsequently detected e.g. via streptavidin or avidin. Pyridyl disulfides can be reacted with sulfhydryl groups to form disulfide bonds via a disulfide exchange reaction. As a result, pyridine-2-thione is released, which can be followed spectrophotometrically (lmax = 343 nm) to monitor the progress of the reaction. The PEG linker improves the overall solubility of the derivative.
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