Cyanine3B methyltetrazine derivative for Inverse electron demand Diels-Alder (IEDDA) conjugation with strained olefins and terminal alkenes. IEDDA is the fastest cycloaddition reaction among click reactions known. Methyltetrazines have optimal physiological pH stability while maintaining extremely high reactivity towards cyclooctenes. Tetrazines also react with some strained cycloalkynes. Cyanine3B is a yellow-emitting cyanine dye. It is an improved version of the Cyanine3 fluorophore, with significantly higher fluorescence quantum yield and photostability.
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