Sulfo-Cyanine5.5 is a dye with far-red emission approaching the NIR range. It has found application in non-invasive live-organism imaging. This particular derivative of sulfo-Cyanine5.5, the DBCO (or ADIBO) derivative, contains cycloalkyne for copper-free conjugation of this fluorophore with various organic azides. The reaction between DBCO and azides is blazingly fast, orders of magnitude exceeding the rate of copper-catalyzed reaction between azides and terminal alkynes. It is also catalyst-free. This reagent can be used to conjugate sulfo-Cyanine5.5 dye to a variety of azide-labeled molecules.
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