APN-PEG4-PFP is a heterobifunctional PEG crosslinker featuring an APN moiety with exceptional cysteine selectivity and a PFP-activated ester for amine conjugation. The PFP esters have similar applications to the NHS esters, but are more stable in aqueous solution. The PEG4 chain elevates water solubility, reduces steric hindrance, and minimizes aggregations. Its highly stable APN–cysteine conjugates withstand aqueous media, human plasma, and live-cell conditions, making it an ideal tool for robust bioconjugation, protein engineering, and therapeutic development.
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