DBCO-PEG4-S-S-PEG4-DBCO possesses two terminal DBCO moieties, each reacting with azide-bearing compounds or biomolecules without the need for a copper catalyst. The PEG4 spacer enhances the hydrophilicity of the molecule. The disulfide bond links cysteines, reducing unfolded protein flexibility and stabilizing structure by ensuring proper folding and strong covalent integrity.
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