AF 594 tyramide, a red fluorescent dye, is integral to tyramide signal amplification (TSA), enhancing fluorescence in immunohistochemistry, immunocytochemistry, and fluorescence in situ hybridization (FISH). TSA utilizes horseradish peroxidase (HRP) to convert tyramine-containing substrates into reactive radicals that covalently bind to nearby proteins. AF 594 tyramide can be used with any HRP-conjugated molecules to stain cells and tissues in immunofluorescence assays.
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