TCO-Amino-Tri-(Acid-ethoxymethyl) is a multifunctional PEG linker containing three carboxylic acids and a trans-cyclooctene (TCO) moiety joined together at a quaternary carbon atom. The carboxylic acids are reactive towards nucleophiles such as amines, and alcohols while the TCO function is applied towards click chemistry with tetrazine-containing compounds. The half-life of TCO is short as it will naturally isomerize into CCO (cis-cycloctene) and lose the reactivity. TCO compound is not recommended for long term storage.
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