Fmoc-Lys(AMCA)-OH is a fluroescent molecule with a Boc protecting group and terminal carboxylic acid and Fmoc moieties. The protected amine can be deprotected under mild acidic conditions. It has an excitation at 345 nm and emission at 450 nm with a relatively large Stoke’s shift and resistance to photobleaching. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. The Fmoc group can be deprotected under basic conditions to obtain the free amine which can be used for further conjugations.
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