Biotin-PEG-CH2CO2H, MW 2,000 can strongly bind with protein, such as avidin or streptavidin, via formation of biotin-protein complex. This biotinylation polymer is used for non-radioactive labeling as well as purification of proteins and other target molecules. The carboxylic acid is reactive with amine in the presence of activator, such as HATU or EDC, to generate a covalent amide linkage.
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