5-TAMRA NHS Ester
Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

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Catalog:
BP-25603
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Name:
5-TAMRA NHS Ester
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Formula:
C29H25N3O7
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MW:
527.5
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CAS:
150810-68-7
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Purity:
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Ships Within:
24 Hours
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Storage Condition:
-20°C
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Excitation Maximum:
553
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Solubility:
DMSO, DMF, MeOH
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Extinction Coefficient Excitation Maximum:
92000
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Emission Maximum:
575
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Shipping:
Ambient temperature
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NMR:
View
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SDS:
View
Product Citations
- van Neer, R. H., Dranchak, P. K., Aitha, M., Liu, L., Carlson, E. K., Jacobsen, I. E., ... & Inglese, J. (2025). Active-and Allosteric-Site Cyclic Peptide Inhibitors of Secreted M. tuberculosis Chorismate Mutase. ACS Infectious Diseases.
https://pubs.acs.org/doi/abs/10.1021/acsinfecdis.4c00798