TCO MagBeads conjugation kit

Superparamagnetic particles that can be used for biomolecule separation, imaging and diagnostic application. BroadPharm provide TCO (trans-cycloctene) MagBeads Kit to convert the amine groups of the MagBeads to TCO groups. The kit contains sufficient reagents & components for performing at least 4 conjugation reactions. Briefly, the amine MagBeads are converted to TCO MagBeads using our TCO-PEG4-NHS ester linker (Scheme 1). Then, the fresh TCO MagBeads are ready to conjugate with the Tetrazine group grafted in antibodies, proteins, peptides, oligonucleotides or other biomolecules via TCO-Tetrazine click chemistry. The TCO click chemistry is the 3rd generation click chemistry that is widely used in labeling and bioconjugation due to its fast speed and high selectivity. The reaction between TCO and Tetrazine moiety is an inverse electron demand Diels Alder (IEDDA) reaction followed by a retro-DA reaction to eliminate nitrogen. Both TCO and Tetrazine moieties are stable in various physiological conditions. With large surface area, excellent colloidal stability and unique surface coating, the functionalized MagBeads exhibit high binding capacity and low non-specific binding of protein or nucleic acids. The functionalized MagBeads are suitable for bioimaging, magnetic separation of specific cellular, protein, bacterial, RNA/DNA and other biomolecules. This product is for Research Use Only. Not for use in diagnostic procedures.

Product Details
Protein Concentration	10 mg/mL
Storage Condition	DI water, 0.05% NaN3, 0.01% tween 20 buffer, 2oC --- 8oC. Do not freeze.
Storage Buffer	50mM Sodium Phosphate buffer pH7.0, 10mL