Site-Specific Streptavidin Antibody Labeling Kit

MAGICLINK™ Site Specific Kits provide optimized reagents for labeling antibodies. The most important features of the kits are ultra-fast reaction speed, high conjugation efficiency, and the most stable linkage between antibody and streptavidin on the market to date. Labeling reactions chemiselectively target disulfide bonds for maximum labeling efficiency. The kits provide optimized reagents for conjugating LINK modified streptavidin to antibodies or other proteins with disulfide bonds. Each 100 μg kit reaction is sufficient for labeling 50-200 μg of antibody in 100 μL reaction volumes, and 500 μg kit for 500 - 1000 μg antibody. The MAGIC Site-Specific linker, provided as a controlled excess reagent, reacts with the antibody under mild alkaline pH conditions. The MAGIC-antibody then reacts instantly with LINK-Streptavidin to get the Streptavidin-Antibody conjugate.

Additionally, the kits include LINK-650 dye to determine the number of MAGIC molecules on the activated antibody. Current antibody labeling methods, such as amidation or amine/thiol conversion for maleimide chemistry, produce random labels on the antibody. In some cases, labeling in Fab areas could block antigen binding. With site-specific cysteine/disulfide chemistry, these linkers are inserted to disulfide bonds and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. As expected for any chemical conjugation reaction, the concentration and buffer formulation of the Link-Streptavidin and the antibody need to fall within certain parameters, as detailed in this user guide.