BP Fluor 647-PEG10-azide is a water-soluble, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or BP Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. The azide reacts with strained cyclooctynes such as BCN, DBCO via a copper-free "click chemistry" reaction. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. The PEG10 enhances water-solubilty and fines tunes the biological activity of the labeled products.
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