Biotin-PEG12-TCO is a biotin PEG reagent with a terminal TCO group. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule. TCO enables fast click chemistry with tetrazine under mild conditions. The half-life of TCO is short as it will naturally isomerize into CCO (cis-cycloctene) and lose the reactivity. TCO compound is not recommended for long term storage.
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